12/7/2023 0 Comments Xld agar e coli![]() Several food borne outbreaks have been associated with the consumption of unpasteurized milk. aureus is mostly present in unpasteurized milk and such contaminated milk products cause food poisoning and gastrointestinal illness, which are responsible for broad variation in texture and taste of milk and produce enzymes which can facilitate bacterial attack and proliferation in the body of host. Sometimes diseased animals like cows and buffaloes also secrete some pathogenic microorganisms in their milk. Bacterial gastro-intestinal infection may also caused by addition of contaminated water in milk. Bacterial gastro-intestinal infection is caused by Campylobacter jejuni. Brucellosis is a disease transmitted to humans by animals due to the consumption of raw milk and its products. Several diseases are caused due to consumption of raw milk and its products. Investigation of pathogens in raw milk and its products is an important step in quality assurance of milk and milk related products. Although pasteurized milk is also available in markets, yet raw milk and its products are consumed by a large population of villages. aureus, Salmonella spp., Coxiella burntinii, Campylobacter etc. Bacterial pathogens such as Mycobacterium tuberculosis, S. ![]() Traditional consumption of raw milk poses a serious threat to health of people. Milk is complex ecosystem for various microorganisms as well it is essential component of diet or best medium for bacterial growth. Raw Milk, Staphylococcus aureus, Salmonella sp., Mannitol Salt Agar, Xylose Lysine Deoxycholate Agar (XLD) This quantitative data could be utilized to better establish the appropriate levels of protection for raw milk, dairy products and processing technologies. The occurrence of high percentage of these pathogenic bacteria in raw milk may be linked to its contamination at the time of collection, processing, strorage and distribution. aureus and 98% identity with Salmonella spp. ![]() The 16SrRNA sequence analysis confirmed the results of biochemical and cultural characterization by depicting 99% identity of samples with S. Of the 200 samples tested, 43% were positive for S almonella, while 45% samples were contaminated with S. Biochemically 87 (43%) samples revealed catalase, citrate, H 2S and fermentation tests positive while oxidase, DNase, Indole and urease tests negative, indicating the presence of Salmonella sp. was detected as gram negative rods with pink coloration on gram staining. Biochemically 91 (45%) samples enhibited Catalase, Coagulase, DNase, Urease, Citrate, fermentation tests positive and indole, oxidase and H 2S tests negative with nonmotile character, indicating the presence of Staphylococcus aureus. Staphylococcus aureus exhibited gram-positive character with purple coloration and it was detected as cocci-shaped. Staphylococcus aureus produced yellow colonies with yellow zones on Mannitol salt agar. Selective medium xylose lysine deoxycholate agar (XLD) and Mannitol salt agar were used for the identification of Salmonella sp. A total of 200 raw milk samples were collected from different cities of Pakistan. The final identification was made using 16SrRNA sequence analysis. Primary screening of raw milk samples was done on the basis of morphological, cultural and biochemical techniques. This study aimed to isolate and identify pathogenic bacteria Staphylococcus aureus and Salmonella from raw milk samples of different cities of Pakistan. These bacteria create serious problems for human health. aureus and Salmonella enter in milk and then multiply, after multiplication they become active in causing diseases. Different types of pathogenic bacteria like S. It is a best source of calcium and phosphorus. Milk is important component of human diet including fats, proteins, vitamins and minerals. Received: Ma Accepted: Published: May 22, 2020įood-borne diseases are the main public health problem throughout the world. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). & D., Islamabad, Pakistan 3Department of Wild Life and Management, University of Haripur, Haripur, Pakistan 4Department of Biosciences, International Islamic University, Islamabad, PakistanĬopyright © 2020 by author(s) and Scientific Research Publishing Inc. 1Department of Microbiology, University of Haripur, Haripur, Pakistan 2Pakistan Agricultural Research Council P.
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